{"id":5106,"date":"2026-08-20T09:11:08","date_gmt":"2026-08-20T07:11:08","guid":{"rendered":"http:\/\/localhost\/idiphisa-nueva-web-2\/?page_id=5106"},"modified":"2026-08-20T11:14:00","modified_gmt":"2026-08-20T09:14:00","slug":"laboratorio-biopsia-liquida","status":"publish","type":"page","link":"https:\/\/investigacionpuertadehierro.com\/en\/laboratorio-biopsia-liquida\/","title":{"rendered":"Liquid Biopsy Laboratory"},"content":{"rendered":"\n\n\n<div class=\"wp-block-greenshift-blocks-row gspb_row gspb_row-id-gsbp-3c65ca7\" id=\"gspb_row-id-gsbp-3c65ca7\"><div class=\"gspb_row__content\"> \n<div class=\"wp-block-greenshift-blocks-row-column gspb_row__col--8 gspb_col-id-gsbp-a7f42b5\" id=\"gspb_col-id-gsbp-a7f42b5\"><h2 class=\"wp-block-post-title has-text-align-left\">Laboratorio Biopsia L\u00edquida<\/h2><\/div>\n <\/div><\/div>\n\n\n\n<div class=\"wp-block-stackable-tabs stk-block-tabs stk-block stk-1fa08ec\" data-initial-tab=\"1\" data-block-id=\"1fa08ec\"><style>.stk-1fa08ec .stk-block-tab-content .stk-block-content .stk-block-column[hidden]{display:none !important;}.stk-1fa08ec {margin-bottom:50px !important;}<\/style><div class=\"stk-row stk-inner-blocks stk-block-content stk-block-tabs--horizontal stk-block-tabs--fade stk-content-align stk-1fa08ec-column\">\n<div class=\"wp-block-stackable-tab-labels stk-block-tab-labels stk-block stk-75e000b stk-block-background is-style-centered-pills\" data-block-id=\"75e000b\"><style>.stk-block-content.stk-block-tabs--horizontal .stk-75e000b{--tabs-flex:1 1 auto !important;}.stk-75e000b .stk-block-tabs__tab{justify-content:flex-start !important;color:#999999 !important;padding-top:8px !important;padding-right:24px !important;padding-bottom:8px !important;padding-left:24px !important;border-top-left-radius:50px !important;border-top-right-radius:50px !important;border-bottom-right-radius:50px !important;border-bottom-left-radius:50px !important;}.stk-75e000b .stk-block-tabs__tab.stk-block-tabs__tab--active .stk-block-tab-labels__text{color:#ffffff !important;}.stk-75e000b {border-top-left-radius:50px !important;border-top-right-radius:50px !important;border-bottom-right-radius:50px !important;border-bottom-left-radius:50px !important;overflow:hidden !important;padding-top:8px !important;padding-right:8px !important;padding-bottom:8px !important;padding-left:8px !important;margin-right:auto !important;margin-left:auto !important;}.stk-75e000b .stk-block-tab-labels__text{color:#999999 !important;}.stk-75e000b .stk-block-tabs__tab:not(.stk-block-tabs__tab--active):hover{background:#dddddd !important;opacity:1 !important;}.stk-75e000b .stk-block-tabs__tab .stk--inner-svg svg:last-child, .stk-75e000b .stk-block-tabs__tab .stk--inner-svg svg:last-child :is(g, path, rect, polygon, ellipse){fill:#777777 !important;}.stk-75e000b .stk-block-tabs__tab:not(.stk-block-tabs__tab--active):hover .stk--inner-svg svg:last-child, .stk-75e000b .stk-block-tabs__tab:not(.stk-block-tabs__tab--active):hover .stk--inner-svg svg:last-child :is(g, path, rect, polygon, ellipse){fill:#000000 !important;}.stk-75e000b .stk-block-tabs__tab.stk-block-tabs__tab--active{background:#000000 !important;}.stk-75e000b .stk-block-tabs__tab.stk-block-tabs__tab--active .stk--inner-svg svg:last-child, .stk-75e000b .stk-block-tabs__tab.stk-block-tabs__tab--active .stk--inner-svg svg:last-child :is(g, path, rect, polygon, ellipse){fill:#ffffff !important;}<\/style><div class=\"stk-block-tab-labels__wrapper\" role=\"tablist\"><button class=\"stk-block-tabs__tab\" role=\"tab\"><span class=\"stk--svg-wrapper\"><div class=\"stk--inner-svg\"><svg style=\"height:0;width:0\"><defs><linearGradient id=\"linear-gradient-75e000b\" x1=\"0\" x2=\"100%\" y1=\"0\" y2=\"0\"><stop offset=\"0%\" style=\"stop-opacity:1;stop-color:var(--linear-gradient-75-e-000-b-color-1)\"><\/stop><stop offset=\"100%\" style=\"stop-opacity:1;stop-color:var(--linear-gradient-75-e-000-b-color-2)\"><\/stop><\/linearGradient><\/defs><\/svg><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 448 512\" aria-hidden=\"true\" width=\"32\" height=\"32\"><path d=\"M288 0H160 128C110.3 0 96 14.3 96 32s14.3 32 32 32V196.8c0 11.8-3.3 23.5-9.5 33.5L10.3 406.2C3.6 417.2 0 429.7 0 442.6C0 480.9 31.1 512 69.4 512H378.6c38.3 0 69.4-31.1 69.4-69.4c0-12.8-3.6-25.4-10.3-36.4L329.5 230.4c-6.2-10.1-9.5-21.7-9.5-33.5V64c17.7 0 32-14.3 32-32s-14.3-32-32-32H288zM192 196.8V64h64V196.8c0 23.7 6.6 46.9 19 67.1L309.5 320h-171L173 263.9c12.4-20.2 19-43.4 19-67.1z\"\/><\/svg><\/div><\/span><div class=\"stk-block-tab-labels__text has-text-align-left\"><span>Laboratorio de Biopsia L\u00edquida<\/span><\/div><\/button><button class=\"stk-block-tabs__tab\" role=\"tab\"><span class=\"stk--svg-wrapper\"><div class=\"stk--inner-svg\"><svg style=\"height:0;width:0\"><defs><linearGradient id=\"linear-gradient-75e000b\" x1=\"0\" x2=\"100%\" y1=\"0\" y2=\"0\"><stop offset=\"0%\" style=\"stop-opacity:1;stop-color:var(--linear-gradient-75-e-000-b-color-1)\"><\/stop><stop offset=\"100%\" style=\"stop-opacity:1;stop-color:var(--linear-gradient-75-e-000-b-color-2)\"><\/stop><\/linearGradient><\/defs><\/svg><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 576 512\" aria-hidden=\"true\" width=\"32\" height=\"32\"><path d=\"M290.8 48.6l78.4 29.7L288 109.5 206.8 78.3l78.4-29.7c1.8-.7 3.8-.7 5.7 0zM136 92.5V204.7c-1.3 .4-2.6 .8-3.9 1.3l-96 36.4C14.4 250.6 0 271.5 0 294.7V413.9c0 22.2 13.1 42.3 33.5 51.3l96 42.2c14.4 6.3 30.7 6.3 45.1 0L288 457.5l113.5 49.9c14.4 6.3 30.7 6.3 45.1 0l96-42.2c20.3-8.9 33.5-29.1 33.5-51.3V294.7c0-23.3-14.4-44.1-36.1-52.4l-96-36.4c-1.3-.5-2.6-.9-3.9-1.3V92.5c0-23.3-14.4-44.1-36.1-52.4l-96-36.4c-12.8-4.8-26.9-4.8-39.7 0l-96 36.4C150.4 48.4 136 69.3 136 92.5zM392 210.6l-82.4 31.2V152.6L392 121v89.6zM154.8 250.9l78.4 29.7L152 311.7 70.8 280.6l78.4-29.7c1.8-.7 3.8-.7 5.7 0zm18.8 204.4V354.8L256 323.2v95.9l-82.4 36.2zM421.2 250.9c1.8-.7 3.8-.7 5.7 0l78.4 29.7L424 311.7l-81.2-31.1 78.4-29.7zM523.2 421.2l-77.6 34.1V354.8L528 323.2v90.7c0 3.2-1.9 6-4.8 7.3z\"\/><\/svg><\/div><\/span><div class=\"stk-block-tab-labels__text has-text-align-left\"><span>Cartera de Servicios<\/span><\/div><\/button><button class=\"stk-block-tabs__tab\" role=\"tab\"><span class=\"stk--svg-wrapper\"><div class=\"stk--inner-svg\"><svg style=\"height:0;width:0\"><defs><linearGradient id=\"linear-gradient-75e000b\" x1=\"0\" x2=\"100%\" y1=\"0\" y2=\"0\"><stop offset=\"0%\" style=\"stop-opacity:1;stop-color:var(--linear-gradient-75-e-000-b-color-1)\"><\/stop><stop offset=\"100%\" style=\"stop-opacity:1;stop-color:var(--linear-gradient-75-e-000-b-color-2)\"><\/stop><\/linearGradient><\/defs><\/svg><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 576 512\" aria-hidden=\"true\" width=\"32\" height=\"32\"><path d=\"M208 80c0-26.5 21.5-48 48-48h64c26.5 0 48 21.5 48 48v64c0 26.5-21.5 48-48 48h-8v40H464c30.9 0 56 25.1 56 56v32h8c26.5 0 48 21.5 48 48v64c0 26.5-21.5 48-48 48H464c-26.5 0-48-21.5-48-48V368c0-26.5 21.5-48 48-48h8V288c0-4.4-3.6-8-8-8H312v40h8c26.5 0 48 21.5 48 48v64c0 26.5-21.5 48-48 48H256c-26.5 0-48-21.5-48-48V368c0-26.5 21.5-48 48-48h8V280H112c-4.4 0-8 3.6-8 8v32h8c26.5 0 48 21.5 48 48v64c0 26.5-21.5 48-48 48H48c-26.5 0-48-21.5-48-48V368c0-26.5 21.5-48 48-48h8V288c0-30.9 25.1-56 56-56H264V192h-8c-26.5 0-48-21.5-48-48V80z\"\/><\/svg><\/div><\/span><div class=\"stk-block-tab-labels__text has-text-align-left\"><span>Funcionamiento  y Estructura<\/span><\/div><\/button><button class=\"stk-block-tabs__tab\" role=\"tab\"><span class=\"stk--svg-wrapper\"><div class=\"stk--inner-svg\"><svg style=\"height:0;width:0\"><defs><linearGradient id=\"linear-gradient-75e000b\" x1=\"0\" x2=\"100%\" y1=\"0\" y2=\"0\"><stop offset=\"0%\" style=\"stop-opacity:1;stop-color:var(--linear-gradient-75-e-000-b-color-1)\"><\/stop><stop offset=\"100%\" style=\"stop-opacity:1;stop-color:var(--linear-gradient-75-e-000-b-color-2)\"><\/stop><\/linearGradient><\/defs><\/svg><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 512 512\" aria-hidden=\"true\" width=\"32\" height=\"32\"><path d=\"M168 80c-13.3 0-24 10.7-24 24V408c0 8.4-1.4 16.5-4.1 24H440c13.3 0 24-10.7 24-24V104c0-13.3-10.7-24-24-24H168zM72 480c-39.8 0-72-32.2-72-72V112C0 98.7 10.7 88 24 88s24 10.7 24 24V408c0 13.3 10.7 24 24 24s24-10.7 24-24V104c0-39.8 32.2-72 72-72H440c39.8 0 72 32.2 72 72V408c0 39.8-32.2 72-72 72H72zM176 136c0-13.3 10.7-24 24-24h96c13.3 0 24 10.7 24 24v80c0 13.3-10.7 24-24 24H200c-13.3 0-24-10.7-24-24V136zm200-24h32c13.3 0 24 10.7 24 24s-10.7 24-24 24H376c-13.3 0-24-10.7-24-24s10.7-24 24-24zm0 80h32c13.3 0 24 10.7 24 24s-10.7 24-24 24H376c-13.3 0-24-10.7-24-24s10.7-24 24-24zM200 272H408c13.3 0 24 10.7 24 24s-10.7 24-24 24H200c-13.3 0-24-10.7-24-24s10.7-24 24-24zm0 80H408c13.3 0 24 10.7 24 24s-10.7 24-24 24H200c-13.3 0-24-10.7-24-24s10.7-24 24-24z\"\/><\/svg><\/div><\/span><div class=\"stk-block-tab-labels__text has-text-align-left\"><span>Producci\u00f3n Cient\u00edfica<\/span><\/div><\/button><\/div><\/div>\n\n\n\n<div class=\"wp-block-stackable-tab-content stk-block-tab-content stk-block stk-b4c5087\" data-block-id=\"b4c5087\"><div class=\"stk-row stk-inner-blocks stk-block-content stk-content-align stk-b4c5087-column\">\n<div class=\"wp-block-stackable-column stk-block-column stk-column stk-block stk-b8a50db\" data-v=\"4\" role=\"tabpanel\" data-block-id=\"b8a50db\"><div class=\"stk-column-wrapper stk-block-column__content stk-container stk-b8a50db-container stk--no-background stk--no-padding\"><div class=\"stk-block-content stk-inner-blocks stk-b8a50db-inner-blocks\">\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-d36162b4 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-container-core-column-is-layout-c3751f7e wp-block-column-is-layout-flow\" style=\"flex-basis:25%\">\n<figure class=\"wp-block-image aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"200\" height=\"267\" src=\"https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/08\/BetetaII-200x267-1.jpg\" alt=\"\" class=\"wp-image-5111\" style=\"object-fit:contain\"\/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-constrained wp-container-core-column-is-layout-b1825af9 wp-block-column-is-layout-constrained\" style=\"padding-top:0px;padding-right:0px;padding-bottom:0px;padding-left:0px;flex-basis:75%\">\n<p class=\"has-text-align-left alignfull wp-block-paragraph\">El Laboratorio de Biopsia L\u00edquida (LBL), del Departamento de Oncolog\u00eda M\u00e9dica, del Hospital Puerta de Hierro, proporciona servicios de diagn\u00f3stico y realiza proyectos de investigaci\u00f3n en el campo de la medicina personalizada. Adem\u00e1s, el laboratorio forma parte de las Unidades comunes del Instituto de Investigaci\u00f3n Sanitaria Puerta de Hierro Segovia de Arana. El laboratorio est\u00e1 registrado en la REDLAB de la Direcci\u00f3n General de Investigaci\u00f3n e Innovaci\u00f3n Tecnol\u00f3gica de la Comunidad de Madrid con n\u00famero de registro 419. El LBL es un laboratorio de referencia para el estudio de mutaciones som\u00e1ticas (del tumor) de forma no invasiva, estando especializado en pacientes con c\u00e1ncer de pulm\u00f3n. De esta manera, LBL recibe muestras de hospitales de toda Espa\u00f1a, participando en varios ensayos cl\u00ednicos del Grupo Espa\u00f1ol de C\u00e1ncer de Pulm\u00f3n. El laboratorio recibe muestras de sangre de pacientes internos, as\u00ed como de otros hospitales ubicados en la comunidad de Madrid para el diagn\u00f3stico de s\u00edndromes del c\u00e1ncer hereditario mediante t\u00e9cnicas de secuenciaci\u00f3n masiva.<\/p>\n<\/div>\n<\/div>\n\n\n\n<p class=\"wp-block-paragraph\">Comprometido a proporcionar pruebas gen\u00e9ticas de alta calidad, LBL participa en varios esquemas de Evaluaci\u00f3n de Calidad Externa (EQA) organizados por la Red Europea de Calidad de Gen\u00e9tica Molecular (EMQN). El laboratorio ha acreditado la mayor\u00eda de sus pruebas NGS de acuerdo con ISO15189. LBL usa tanto plataformas Illumia como Ion (Thermofisher). LBL colabora en varios proyectos europeos de la convocatoria H2020 y forma parte del consorcio internacional BCAC. Adem\u00e1s, el LBL lidera varios proyectos de investigaci\u00f3n financiados por convocatorias nacionales de concurrencia competitiva, y tiene una extraordinaria producci\u00f3n cient\u00edfica publicando en revistas de alto impacto.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">El laboratorio recibe estudiantes de diversas Universidades nacionales o internacionales. Del mismo modo recibe residentes de oncolog\u00eda, bioqu\u00edmica cl\u00ednica y an\u00e1lisis cl\u00ednicos de diversos hospitales de \u00e1mbito nacional.<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"150\" src=\"https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/08\/Mapa-lab-biopsia-liquida-300x150-1.jpg\" alt=\"\" class=\"wp-image-5112\" style=\"aspect-ratio:2;width:300px;height:auto\"\/><\/figure>\n<\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-stackable-column stk-block-column stk-column stk-block stk-502b907\" data-v=\"4\" role=\"tabpanel\" data-block-id=\"502b907\"><div class=\"stk-column-wrapper stk-block-column__content stk-container stk-502b907-container stk--no-background stk--no-padding\"><div class=\"stk-block-content stk-inner-blocks stk-502b907-inner-blocks\">\n<p class=\"wp-block-paragraph\">El estudio de biomarcadores en biopsia l\u00edquidas permite identificar mutaciones som\u00e1ticas, del tumor, que son diana terap\u00e9uticas de ciertos f\u00e1rmacos dirigidos, en el plasma sangu\u00edneo de los pacientes, siendo una t\u00e9cnica m\u00ednimamente invasiva.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><u>Tiempo de respuesta<\/u>.<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>El tiempo m\u00e1ximo de respuestas ser\u00e1 de 12 d\u00edas naturales.<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><u>Especificaciones del paciente.<\/u><\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Dieta previa a la prueba<\/strong>. El paciente puede mantener su dieta habitual.<\/li>\n\n\n\n<li><strong>Supresi\u00f3n de f\u00e1rmacos anterior a la prueba.<\/strong>&nbsp;No es necesario suspender la medicaci\u00f3n.<\/li>\n\n\n\n<li><strong>Periodo de ayuno anterior a la prueba.&nbsp;&nbsp;<\/strong>No es necesario el ayuno para la extracci\u00f3n.<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><u>Especificaciones del esp\u00e9cimen.<\/u><\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Tipo de esp\u00e9cimen.<\/strong>&nbsp;Sangre perif\u00e9rica.<\/li>\n\n\n\n<li><strong>Tipo de contenedor.<\/strong>&nbsp;Son v\u00e1lidos los tubos que aparecen en la IT-001 (Tubo PTT EDTA K2 (BECTON .DICKINSON) con gel separador, Tubo PAXgene Blood ccfDNA, Cell-Free DNA BCT Streck)<\/li>\n\n\n\n<li><strong>Volumen m\u00ednimo.&nbsp;&nbsp;<\/strong>Siempre que las condiciones del paciente lo permitan se intentar\u00e1 recoger un volumen de entre 7 y 10 ml. Las muestras con menos de 7 ml se recoger\u00e1n de igual forma y ser\u00e1 el Responsable del Laboratorio de Biopsia L\u00edquida-Oncolog\u00eda M\u00e9dica el encargado de valorar las condiciones de la muestra.<\/li>\n\n\n\n<li><strong>Recomendaciones especiales para el transporte.<\/strong>&nbsp;Se recomienda transportar la muestra tan pronto como sea posible para evitar la contaminaci\u00f3n por DNA gen\u00f3mico.<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><u>Especificaciones anal\u00edticas.<\/u><\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>M\u00e9todo de an\u00e1lisis.<\/strong>&nbsp;Detecci\u00f3n y cuantificaci\u00f3n de ADN tumoral circulante mediante PCR digital basada en arrays.<\/li>\n\n\n\n<li><strong>Instrumentaci\u00f3n.<\/strong>&nbsp;PCR digital de Thermo Scientific.<\/li>\n\n\n\n<li><strong>Especificaciones del par\u00e1metro.&nbsp;<\/strong>Se considerar\u00e1 positivo todo aquel resultado por encima del l\u00edmite de detecci\u00f3n de la prueba, establecido en 0.1%.<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>DIAGN\u00d3STICO MOLECULAR DE C\u00c1NCER FAMILIAR<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Mediante esta prueba se pueden diagnosticar los siguientes s\u00edndromes en familias de alto riesgo:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Diagn\u00f3stico del s\u00edndrome de c\u00e1ncer de mama y ovario hereditario (genes ATM, BARD1, BRIP1, BRCA1, BRCA2, CHEK2, PALB2, RAD51C, RAD51D)<\/li>\n\n\n\n<li><a href=\"https:\/\/investigacionpuertadehierro.com\/calculadora-poliposis\/\">Diagn\u00f3stico de la Poliposis&nbsp;Adenomatosa Familiar (genes&nbsp;APC, AXIN2,&nbsp; MUTYH, NTHL1, POLD1, POLE)<\/a><\/li>\n\n\n\n<li>S\u00edndrome de poliposis juvenil (genes&nbsp;BMPR1A,&nbsp;SMAD4)<\/li>\n\n\n\n<li>Diagn\u00f3stico del S\u00edndrome&nbsp;de Lynch (genes EPCAM, MLH1, MSH2, MSH6, PMS2)<\/li>\n\n\n\n<li>C\u00e1ncer g\u00e1strico difuso hereditario (genes CDH1, CTNNA1)<\/li>\n\n\n\n<li>S\u00edndrome de Li-Fraumeni (gen TP53)<\/li>\n\n\n\n<li>Melanoma familiar (genes&nbsp;BAP1,&nbsp;CDKN2A, CDK4, MC1R, POT1, TERF2IP, ACD, TERT)<\/li>\n\n\n\n<li>S\u00edndrome de Peutz-Jeghers (gen STK11)<\/li>\n\n\n\n<li>Leiomiomatosis hereditaria (gen FH)<\/li>\n\n\n\n<li>Feocromocitoma-paraganglioma hereditario (genes SDHA, SDHAF2, SDHB, SDHC, SDHD, TMEM127)<\/li>\n\n\n\n<li>Neoplasias endocrinas m\u00faltiples (genes MEN1 y RET)<\/li>\n\n\n\n<li>Diagn\u00f3stico del s\u00edndrome de von Hippel-Lindau (gen VHL)<\/li>\n\n\n\n<li>Carcinoma renal papilar hereditario (gen MET)<\/li>\n\n\n\n<li>P\u00e1ncreas hereditario (genes PRSS1, BRCA1, BRCA2, PALB2, ATM, CDKN2A)<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">Tambi\u00e9n permite identificar a los pacientes candidatos a recibir un f\u00e1rmaco inhibidor de PARP. El laboratorio est\u00e1 acreditado conforme a la norma UNE-EN ISO 15189 para el estudio de mutaciones en los siguientes genes:<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">ACD, AIP, AKT1, ATM, AXIN2, BAP1, BARD1, BMPR1A, BRIP1, CDH1, CDK4, CDKN2A, CHEK2, CTNNA1, DICER1, FAM175A, FH, FLCN, GREM1, HABP2, HOXB13, KIF1B, MAX, MC1R, MEN1, MET, MITF, MLH3, MRE11, MSH3, NBM, NF1, NF2, NTHL1, PALB2, PIK3CA, POLD1, POLE, POT1, PRKAR1A, PRSS1, PTCH1, PTEN, RAD50, RAD51C, RAD51D, RB1, RET, SDHA, SDHAF2, SDHC, SDHS, SLC45A2, SMAD4, SMARCB1, STK11, SUFU, TERF2IP, TERT, TMEM127, TP53, VHL y XRCC2.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Tiempo de respuesta.<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>El tiempo aproximado de respuesta ser\u00e1 entre 6 y 8 meses.<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><u>Especificaciones del paciente.<\/u><\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Dieta previa a la prueba<\/strong>. El paciente puede mantener su dieta habitual.<\/li>\n\n\n\n<li><strong>Supresi\u00f3n de f\u00e1rmacos anterior a la prueba.<\/strong>&nbsp;No es necesario suspender la medicaci\u00f3n.<\/li>\n\n\n\n<li><strong>Periodo de ayuno anterior a la prueba.&nbsp;&nbsp;<\/strong>No es necesario el ayuno para la extracci\u00f3n.<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><u>Especificaciones del esp\u00e9cimen.<\/u><\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Tipo de esp\u00e9cimen.<\/strong>&nbsp;Sangre perif\u00e9rica.<\/li>\n\n\n\n<li><strong>Tipo de contenedor.<\/strong>&nbsp;Tubo hematolog\u00eda EDTA.<\/li>\n\n\n\n<li><strong>Volumen m\u00ednimo.&nbsp;&nbsp;<\/strong>Siempre que las condiciones del paciente lo permitan se intentar\u00e1 recoger un volumen de entre 7 y 10 ml. Las muestras con menos de 7 ml se recoger\u00e1n de igual forma y ser\u00e1 el Responsable del Laboratorio de Biopsia L\u00edquida-Oncolog\u00eda M\u00e9dica el encargado de valorar las condiciones de la muestra.<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><u>Especificaciones anal\u00edticas<\/u><\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>M\u00e9todo de an\u00e1lisis.<\/strong>&nbsp;Detecci\u00f3n de mutaciones en los genes de susceptibilidad al c\u00e1ncer por Secuenciaci\u00f3n masiva\/Sanger. Detecci\u00f3n de grandes reordenamientos por electroforesis capilar.<\/li>\n\n\n\n<li><strong>Instrumentaci\u00f3n.<\/strong>&nbsp;Termocicladores\/Secuenciador Sanger\/Secuenciador Masivo.<\/li>\n<\/ul>\n<\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-stackable-column stk-block-column stk-column stk-block stk-559faaf\" data-v=\"4\" role=\"tabpanel\" data-block-id=\"559faaf\"><div class=\"stk-column-wrapper stk-block-column__content stk-container stk-559faaf-container stk--no-background stk--no-padding\"><div class=\"stk-block-content stk-inner-blocks stk-559faaf-inner-blocks\">\n<div id=\"funcionamiento\"><\/div><h5 id=\"gspb_heading-id-gsbp-cd2416d\" class=\"gspb_heading gspb_heading-id-gsbp-cd2416d \">ORGANIGRAMA<\/h5>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1011\" height=\"701\" src=\"https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/08\/Organigrama-LBL.png\" alt=\"\" class=\"wp-image-5116\" style=\"aspect-ratio:1.440922190201729;object-fit:cover;width:600px;height:auto\" srcset=\"https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/08\/Organigrama-LBL.png 1011w, https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/08\/Organigrama-LBL-767x532.png 767w, https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/08\/Organigrama-LBL-300x208.png 300w\" sizes=\"auto, (max-width: 1011px) 100vw, 1011px\" \/><\/figure>\n\n\n\n<div id=\"funcionamiento\"><\/div><h5 id=\"gspb_heading-id-gsbp-74d1d56\" class=\"gspb_heading gspb_heading-id-gsbp-74d1d56 \">DIRECCI\u00d3N T\u00c9CNICA<\/h5>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><em><u>Responsable del Laboratorio de Biopsia L\u00edquida<\/u><\/em><\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Nombre y Apellidos<\/strong>: Atocha Romero Alfonso<\/li>\n\n\n\n<li><strong>email<\/strong>:&nbsp;<a href=\"mailto:aromero@idiphim.org\">aromero@idiphim.org<\/a>; atocha.romero@salud.madrid.org.<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><em><u>Jefe de Servicio de Oncolog\u00eda M\u00e9dica:<\/u><\/em><\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Nombre y Apellidos<\/strong>: Mariano Provencio Pulla<\/li>\n\n\n\n<li><strong>email<\/strong>: mariano.provencio@salud.madrid.org<\/li>\n<\/ul>\n\n\n\n<div id=\"funcionamiento\"><\/div><h5 id=\"gspb_heading-id-gsbp-f377e3e\" class=\"gspb_heading gspb_heading-id-gsbp-f377e3e \">RESPONSABLE DEL SISTEMA DE GESTI\u00d3N DE CALIDAD<\/h5>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Nombre y Apellidos<\/strong>: Roberto Serna Blasco<\/li>\n\n\n\n<li><strong>Soporte<\/strong>: Unidad de Calidad del Instituto de Investigaci\u00f3n Sanitaria Puerta de Hierro<\/li>\n<\/ul>\n\n\n\n<div id=\"funcionamiento\"><\/div><h5 id=\"gspb_heading-id-gsbp-3c685ab\" class=\"gspb_heading gspb_heading-id-gsbp-3c685ab \">PERSONAL DEL LABORATORIO<\/h5>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Nombre y Apellidos<\/strong>: Pilar Mediavilla Medel.&nbsp;<strong>Puesto<\/strong>: Personal de Laboratorio con titulaci\u00f3n superior<\/li>\n\n\n\n<li><strong>Nombre y Apellidos<\/strong>: Roberto Serna Blasco.&nbsp;<strong>Puesto<\/strong>: Personal de Laboratorio con titulaci\u00f3n superior<\/li>\n\n\n\n<li><strong>Nombre y Apellidos<\/strong>: Luc\u00eda Robado de Lope.&nbsp;<strong>Puesto<\/strong>: Personal de Laboratorio con titulaci\u00f3n superior<\/li>\n\n\n\n<li><strong>Nombre y Apellidos<\/strong>: Natalia Garc\u00eda Sim\u00f3n.&nbsp;<strong>Puesto:&nbsp;<\/strong>Personal de Laboratorio con titulaci\u00f3n superior<\/li>\n\n\n\n<li><strong>Nombre y Apellidos<\/strong>: Eva de la Torre Capit\u00e1n Mat\u00edas.&nbsp;<strong>Puesto:&nbsp;<\/strong>Personal de Laboratorio con titulaci\u00f3n superior<\/li>\n\n\n\n<li><strong>Nombre y Apellidos<\/strong>: Leonor Octavio Gonz\u00e1lez.&nbsp;<strong>Puesto:&nbsp;<\/strong>Personal de Laboratorio con titulaci\u00f3n superior<\/li>\n\n\n\n<li><strong>Nombre y Apellidos<\/strong>: Sandra Sanz Moreno.&nbsp;<strong>Puesto<\/strong>: T\u00e9cnico de laboratorio<\/li>\n\n\n\n<li><strong>Nombre y Apellidos<\/strong>: Alejandro Rodr\u00edguez Festa.&nbsp;<strong>Puesto:&nbsp;<\/strong>T\u00e9cnico de laboratorio<strong>.<\/strong><\/li>\n\n\n\n<li><strong>Nombre y Apellidos<\/strong>: Patricia Gallego Gil.&nbsp;<strong>Puesto:&nbsp;<\/strong>T\u00e9cnico de laboratorio<strong>.<\/strong><\/li>\n\n\n\n<li><strong>Nombre y Apellidos<\/strong>: Claudia Gonz\u00e1lez Alcade.&nbsp;<strong>Puesto:&nbsp;<\/strong>T\u00e9cnico de laboratorio<strong>.<\/strong><\/li>\n<\/ul>\n<\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-stackable-column stk-block-column stk-column stk-block stk-6714c39\" data-v=\"4\" role=\"tabpanel\" data-block-id=\"6714c39\"><div class=\"stk-column-wrapper stk-block-column__content stk-container stk-6714c39-container stk--no-background stk--no-padding\"><div class=\"stk-block-content stk-inner-blocks stk-6714c39-inner-blocks\">\n<div id=\"funcionamiento\"><\/div><h5 id=\"gspb_heading-id-gsbp-47f00c3\" class=\"gspb_heading gspb_heading-id-gsbp-47f00c3 \">PROYECTOS EN CURSO<\/h5>\n\n\n\n<ul class=\"wp-block-list\">\n<li>2025-actualidad: Papel de la microbiota intestinal en la respuesta a la quimio-inmunoterapia en c\u00e1ncer de pulm\u00f3n de c\u00e9lulas no peque\u00f1as.&nbsp;<strong>PI24\/01725&nbsp;<\/strong>(ISCIII). Cuant\u00eda:&nbsp;<strong>340.000 \u20ac<\/strong>.&nbsp;<strong>IP<\/strong>:&nbsp;<strong>Atocha Romero.<\/strong><\/li>\n\n\n\n<li>2022-actualidad: Validaci\u00f3n cl\u00ednica de un sistema para detectar translocaciones de ALK en ves\u00edculas extracelulares en plasma III.&nbsp;<strong>DTS21\/00124&nbsp;<\/strong>(ISCIII). Cuant\u00eda:&nbsp;<strong>88.550,00\u20ac&nbsp;<\/strong>(presupuesto exclusivo para el centro de la IP).&nbsp;<strong>IP. Coordinador&nbsp;<\/strong>(estudio multic\u00e9ntrico):&nbsp;<strong>Atocha Romero.<\/strong><\/li>\n\n\n\n<li>2022-actualidad:&nbsp;<em>Biomarkers4Cure<\/em>. Biomarcadores predictivos de respuesta a combinaciones de inmunoterapia m\u00e1s quimioterapia neoadyuvante en pacientes con c\u00e1ncer de pulm\u00f3n operable.&nbsp;<strong>PI21\/01500&nbsp;<\/strong>(ISCIII). Cuant\u00eda:&nbsp;<strong>266.00 \u20ac. IP: Atocha Romero.<\/strong><\/li>\n\n\n\n<li>2020-actualidad: Investigador colaborador en el proyecto RETOS-COLABORACI\u00d3N titulado \u201cBLI-O. Desarrollo de marcadores moleculares en biopsia liquida para larga supervivencia en oncoinmunoterapia (I-O)\u201d.&nbsp;<strong>RTC2019- 007359-<\/strong>1<strong>. IP:Mariano Provencio.<\/strong><\/li>\n\n\n\n<li>2022-actualidad<strong>:&nbsp;<\/strong>Determinaci\u00f3n de mutaciones DDR en una cohorte de pacientes con CPRCm mediante biopsia l\u00edquida (ctDNA). Concordancia con el estudio germinal y en tejido tumoral.&nbsp;<strong>PI 175\/21<\/strong>.&nbsp;<strong>IP: Atocha Romero<\/strong><\/li>\n\n\n\n<li>2023-2025: Quimio-inmunoterapia en estadios localmente avanzados deCPCNP: validaci\u00f3n de marcadores derivados del ctDNA y el microambiente tumoral. Funding body and call: ISCIII (AES 2022). PI22\/01223. Cuant\u00eda:&nbsp;<strong>268.620<\/strong>&nbsp;\u20ac.&nbsp;<strong>I.P: Mariano Provencio (HUPH).<\/strong><\/li>\n<\/ul>\n\n\n\n<div id=\"funcionamiento\"><\/div><h5 id=\"gspb_heading-id-gsbp-81c23ca\" class=\"gspb_heading gspb_heading-id-gsbp-81c23ca \">PUBLICACIONES SELECCIONADAS<\/h5>\n\n\n\n<ol class=\"wp-block-list\">\n<li><a href=\"https:\/\/doi.org\/10.1056\/NEJMoa2215530\">Provencio M, Nadal E, Gonz\u00e1lez-Larriba JL, Mart\u00ednez-Mart\u00ed A, Bernab\u00e9 R, Bosch-Barrera J, Casal-Rubio J, Calvo V, Insa A, Ponce S, Reguart N, de Castro J, Mosquera J, Cobo M, Aguilar A, L\u00f3pez Vivanco G, Camps C, L\u00f3pez-Castro R, Mor\u00e1n T, Barneto I, Rodr\u00edguez-Abreu D, Serna-Blasco R, Ben\u00edtez R, Aguado de la Rosa C, Palmero R, Hernando-Trancho F, Mart\u00edn-L\u00f3pez J, Cruz-Berm\u00fadez A, Massuti B, Romero A. Perioperative Nivolumab and Chemotherapy in Stage III Non-Small-Cell Lung Cancer. N Engl J Med. 2023 Aug 10;389(6):504-513. doi: 10.1056\/NEJMoa2215530. Epub 2023 Jun 28. PMID: 37379158.<img loading=\"lazy\" decoding=\"async\" width=\"30\" height=\"30\" src=\"http:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2019\/06\/icon_paper.jpg\" alt=\"\"><\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/doi.org\/10.1001\/jamaoncol.2022.5959\">Provencio M, et al. Atezolizumab Plus Bevacizumab as First-line Treatment for Patients With Metastatic Nonsquamous Non-Small Cell Lung Cancer With High Tumor Mutation Burden: A Nonrandomized Controlled Trial. 2022. JAMA Oncol. Online ahead of print. doi: 10.1001\/jamaoncol.2022.5959.<img loading=\"lazy\" decoding=\"async\" width=\"30\" height=\"30\" src=\"http:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2019\/06\/icon_paper.jpg\" alt=\"\"><\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/doi.org\/10.1200\/JCO.21.02660\">Provencio M et al. Overall Survival and Biomarker Analysis of Neoadjuvant Nivolumab Plus Chemotherapy in Operable Stage IIIA Non-Small-Cell Lung Cancer (NADIM phase II trial). J Clin Oncol, 40(25):2924 2933. doi: 10.1200\/JCO.21.02660<img loading=\"lazy\" decoding=\"async\" width=\"30\" height=\"30\" src=\"http:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2019\/06\/icon_paper.jpg\" alt=\"\"><\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/doi.org\/10.1093\/clinchem\/hvac021\">S\u00e1nchez-Herrero E, et al. ALK-Fusion Transcripts Can Be Detected in Extracellular Vesicles (EVs) from Nonsmall Cell Lung Cancer Cell Lines and Patient Plasma: Toward EV-Based Noninvasive Testing. Clin Chem. 2022 Mar 28;hvac021.<img loading=\"lazy\" decoding=\"async\" width=\"30\" height=\"30\" src=\"http:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2019\/06\/icon_paper.jpg\" alt=\"\"><\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/doi.org\/10.1158\/1078-0432.CCR-21-1200\">Casarrubios M,et al. Pretreatment Tissue TCR Repertoire Evenness Is Associated with Complete Pathologic Response in Patients with NSCLC Receiving Neoadjuvant Chemoimmunotherapy. 2021. Clin Cancer Res, 27(21):5878-5890. doi: 10.1158\/1078-0432.CCR-21-1200.<img loading=\"lazy\" decoding=\"async\" width=\"30\" height=\"30\" src=\"http:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2019\/06\/icon_paper.jpg\" alt=\"\"><\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/doi.org\/10.1136\/jitc-2021-002804\">Sierra-Rodero B, Cruz-Berm\u00fadez A (CA), Nadal E, \u2026 Provencio M (CA) (26\/26). Clinical and molecular parameters associated to pneumonitis development in non-small-cell lung cancer patients receiving chemoimmunotherapy from NADIM trial. J Immunother Cancer, 9(8): e002804. doi: 10.1136\/jitc-2021-002804.<img loading=\"lazy\" decoding=\"async\" width=\"30\" height=\"30\" src=\"http:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2019\/06\/icon_paper.jpg\" alt=\"\"><\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/doi.org\/10.1002\/1878-0261.13033\">S\u00e1nchez-Herrero E, et al. NGS-based liquid-biopsy profiling identifies mechanisms of resistance to ALK inhibitors: a step towards personalized NSCLC treatment. Mol Oncol. 2021 May 31. doi: 10.1002\/1878-0261.13033<img loading=\"lazy\" decoding=\"async\" width=\"30\" height=\"30\" src=\"http:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2019\/06\/icon_paper.jpg\" alt=\"\"><\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/doi.org\/10.1002\/ctm2.491\">Laza-Briviesca R, et al. Blood biomarkers associated to complete pathological response on NSCLC patients treated with neoadjuvant Chemoimmunotherapy included in NADIM clinical trial. 2021. Clin Transl Med, 11(7):e491. doi: 10.1002\/ctm2.491.<img loading=\"lazy\" decoding=\"async\" width=\"30\" height=\"30\" src=\"http:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2019\/06\/icon_paper.jpg\" alt=\"\"><\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/doi.org\/10.1515\/cclm-2020-1465\">P\u00e9rez-Barrios C, et al. ctDNA from body fluids is an adequate source for EGFR biomarker testing in advanced lung adenocarcinoma. Clin Chem Lab Med. Clin Chem Lab Med. 2021 Mar 11;59(7):1221-1229<img loading=\"lazy\" decoding=\"async\" width=\"30\" height=\"30\" src=\"http:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2019\/06\/icon_paper.jpg\" alt=\"\"><\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/doi.org\/10.1016\/j.ejca.2021.02.031\">Provencio M et al. Analysis of circulating tumour DNA to identify patients with epidermal growth factor receptor positive non-small cell lung cancer who might benefit from sequential tyrosine kinase inhibitor treatment. Eur J Cancer, 149:61-72. doi: 10.1016\/j.ejca.2021.02.031.<img loading=\"lazy\" decoding=\"async\" width=\"30\" height=\"30\" src=\"http:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2019\/06\/icon_paper.jpg\" alt=\"\"><\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/doi.org\/10.1016\/S1470-2045(20)30453-8\">Provencio M et al. Neoadjuvant chemotherapy and nivolumab in resectable non-small-cell lung cancer (NADIM): an open label, multicentre, single-arm, phase 2 trial. 2020. Lancet Oncol, 21(11):1413 1422. doi: 1016\/S1470-2045(20)30453-8.<img loading=\"lazy\" decoding=\"async\" width=\"30\" height=\"30\" src=\"http:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2019\/06\/icon_paper.jpg\" alt=\"\"><\/a><\/li>\n<\/ol>\n<\/div><\/div><\/div>\n<\/div><\/div>\n<\/div><\/div>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Laboratorio de Biopsia L\u00edquida Cartera de Servicios Funcionamiento y Estructura Producci\u00f3n Cient\u00edfica El Laboratorio de Biopsia L\u00edquida (LBL), del Departamento de Oncolog\u00eda M\u00e9dica, del Hospital Puerta de Hierro, proporciona servicios de diagn\u00f3stico y realiza proyectos de investigaci\u00f3n en el campo de la medicina personalizada. Adem\u00e1s, el laboratorio forma parte de las Unidades comunes del Instituto [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_gspb_post_css":"#gspb_row-id-gsbp-3c65ca7{justify-content:space-between;margin-top:0;margin-bottom:0;display:flex;flex-wrap:wrap}#gspb_row-id-gsbp-3c65ca7>.gspb_row__content{display:flex;justify-content:space-between;margin:0 auto;width:100%;flex-wrap:wrap}.gspb_row{position:relative}div[id^=gspb_col-id]{box-sizing:border-box;position:relative;padding:var(--gs-row-column-padding, 15px min(3vw, 20px))}body.gspb-bodyfront #gspb_row-id-gsbp-3c65ca7>.gspb_row__content{width:var(--theme-container-width, 1200px);max-width:var(--theme-normal-container-max-width, 1200px)}#gspb_col-id-gsbp-a7f42b5.gspb_row__col--8{width:66.66666666666667%}@media (max-width:575.98px){#gspb_col-id-gsbp-a7f42b5.gspb_row__col--8{width:100%}}@media (min-width:992px){body.gspb-bodyfront #gspb_col-id-gsbp-a7f42b5.gspb_row__col--8{width:calc(80% - 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