{"id":5070,"date":"2026-08-19T15:35:49","date_gmt":"2026-08-19T13:35:49","guid":{"rendered":"http:\/\/localhost\/idiphisa-nueva-web-2\/?page_id=5070"},"modified":"2026-10-05T12:38:48","modified_gmt":"2026-10-05T10:38:48","slug":"advanced-optical-and-confocal-microscopy-unit","status":"publish","type":"page","link":"https:\/\/investigacionpuertadehierro.com\/en\/unidad-de-microscopia-optica-avanzada-y-confocal\/","title":{"rendered":"Advanced Optical and Confocal Microscopy Unit"},"content":{"rendered":"\n\n\n<div class=\"wp-block-greenshift-blocks-row gspb_row gspb_row-id-gsbp-3c65ca7\" id=\"gspb_row-id-gsbp-3c65ca7\"><div class=\"gspb_row__content\"> \n<div class=\"wp-block-greenshift-blocks-row-column gspb_row__col--4 gspb_col-id-gsbp-6dd98bb\" id=\"gspb_col-id-gsbp-6dd98bb\">\n<div class=\"wp-block-greenshift-blocks-image gspb_image gspb_image-id-gsbp-67a39a3\" id=\"gspb_image-id-gsbp-67a39a3\"><img decoding=\"async\" src=\"https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Logotipo-de-Microscopia-Optica-Avanzada-3.png\" data-src=\"\" alt=\"\" loading=\"lazy\" width=\"1205\" height=\"1305\"\/><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-greenshift-blocks-row-column gspb_row__col--8 gspb_col-id-gsbp-a7f42b5\" id=\"gspb_col-id-gsbp-a7f42b5\"><h2 class=\"wp-block-post-title has-text-align-left\">Unidad de Microscop\u00eda \u00d3ptica Avanzada y Confocal<\/h2><\/div>\n\n\n\n<div class=\"wp-block-greenshift-blocks-row-column gspb_row__col--4 gspb_col-id-gsbp-54b36d4\" id=\"gspb_col-id-gsbp-54b36d4\">\n<div class=\"wp-block-greenshift-blocks-image gspb_image gspb_image-id-gsbp-a3cf4dd\" id=\"gspb_image-id-gsbp-a3cf4dd\"><a href=\"http:\/\/labs.remoa.net\/lab.php?labid=91\" target=\"_blank\" ><img decoding=\"async\" src=\"https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/08\/remoa.jpg\" data-src=\"\" alt=\"\" loading=\"lazy\" width=\"196\" height=\"100\"\/><\/a><\/div>\n<\/div>\n <\/div><\/div>\n\n\n\n<div class=\"wp-block-stackable-tabs stk-block-tabs stk-block stk-1fa08ec\" data-initial-tab=\"1\" data-block-id=\"1fa08ec\"><style>.stk-1fa08ec .stk-block-tab-content .stk-block-content .stk-block-column[hidden]{display:none !important;}.stk-1fa08ec {margin-bottom:50px !important;}<\/style><div class=\"stk-row stk-inner-blocks stk-block-content stk-block-tabs--horizontal stk-block-tabs--fade stk-content-align stk-1fa08ec-column\">\n<div class=\"wp-block-stackable-tab-labels stk-block-tab-labels stk-block stk-75e000b stk-block-background is-style-centered-pills\" data-block-id=\"75e000b\"><style>.stk-block-content.stk-block-tabs--horizontal .stk-75e000b{--tabs-flex:1 1 auto !important;}.stk-75e000b .stk-block-tabs__tab{justify-content:flex-start !important;color:#999999 !important;padding-top:8px !important;padding-right:24px !important;padding-bottom:8px !important;padding-left:24px !important;border-top-left-radius:50px !important;border-top-right-radius:50px !important;border-bottom-right-radius:50px !important;border-bottom-left-radius:50px !important;}.stk-75e000b .stk-block-tabs__tab.stk-block-tabs__tab--active .stk-block-tab-labels__text{color:#ffffff !important;}.stk-75e000b {border-top-left-radius:50px !important;border-top-right-radius:50px !important;border-bottom-right-radius:50px !important;border-bottom-left-radius:50px !important;overflow:hidden !important;padding-top:8px !important;padding-right:8px !important;padding-bottom:8px !important;padding-left:8px !important;margin-right:auto !important;margin-left:auto !important;}.stk-75e000b .stk-block-tab-labels__text{color:#999999 !important;}.stk-75e000b .stk-block-tabs__tab:not(.stk-block-tabs__tab--active):hover{background:#dddddd !important;opacity:1 !important;}.stk-75e000b .stk-block-tabs__tab .stk--inner-svg svg:last-child, .stk-75e000b .stk-block-tabs__tab .stk--inner-svg svg:last-child :is(g, path, rect, polygon, ellipse){fill:#777777 !important;}.stk-75e000b .stk-block-tabs__tab:not(.stk-block-tabs__tab--active):hover .stk--inner-svg svg:last-child, .stk-75e000b .stk-block-tabs__tab:not(.stk-block-tabs__tab--active):hover .stk--inner-svg svg:last-child :is(g, path, rect, polygon, ellipse){fill:#000000 !important;}.stk-75e000b .stk-block-tabs__tab.stk-block-tabs__tab--active{background:#000000 !important;}.stk-75e000b .stk-block-tabs__tab.stk-block-tabs__tab--active .stk--inner-svg svg:last-child, .stk-75e000b .stk-block-tabs__tab.stk-block-tabs__tab--active .stk--inner-svg svg:last-child :is(g, path, rect, polygon, ellipse){fill:#ffffff !important;}<\/style><div class=\"stk-block-tab-labels__wrapper\" role=\"tablist\"><button class=\"stk-block-tabs__tab\" role=\"tab\"><span class=\"stk--svg-wrapper\"><div class=\"stk--inner-svg\"><svg style=\"height:0;width:0\"><defs><linearGradient id=\"linear-gradient-75e000b\" x1=\"0\" x2=\"100%\" y1=\"0\" y2=\"0\"><stop offset=\"0%\" style=\"stop-opacity:1;stop-color:var(--linear-gradient-75-e-000-b-color-1)\"><\/stop><stop offset=\"100%\" style=\"stop-opacity:1;stop-color:var(--linear-gradient-75-e-000-b-color-2)\"><\/stop><\/linearGradient><\/defs><\/svg><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 512 512\" aria-hidden=\"true\" width=\"32\" height=\"32\"><path d=\"M256 512A256 256 0 1 0 256 0a256 256 0 1 0 0 512zM216 336h24V272H216c-13.3 0-24-10.7-24-24s10.7-24 24-24h48c13.3 0 24 10.7 24 24v88h8c13.3 0 24 10.7 24 24s-10.7 24-24 24H216c-13.3 0-24-10.7-24-24s10.7-24 24-24zm40-208a32 32 0 1 1 0 64 32 32 0 1 1 0-64z\"\/><\/svg><\/div><\/span><div class=\"stk-block-tab-labels__text has-text-align-left\"><span>Descripci\u00f3n<\/span><\/div><\/button><button class=\"stk-block-tabs__tab\" role=\"tab\"><span class=\"stk--svg-wrapper\"><div class=\"stk--inner-svg\"><svg style=\"height:0;width:0\"><defs><linearGradient id=\"linear-gradient-75e000b\" x1=\"0\" x2=\"100%\" y1=\"0\" y2=\"0\"><stop offset=\"0%\" style=\"stop-opacity:1;stop-color:var(--linear-gradient-75-e-000-b-color-1)\"><\/stop><stop offset=\"100%\" style=\"stop-opacity:1;stop-color:var(--linear-gradient-75-e-000-b-color-2)\"><\/stop><\/linearGradient><\/defs><\/svg><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 512 512\" aria-hidden=\"true\" width=\"32\" height=\"32\"><path d=\"M256 512A256 256 0 1 0 256 0a256 256 0 1 0 0 512zM216 336h24V272H216c-13.3 0-24-10.7-24-24s10.7-24 24-24h48c13.3 0 24 10.7 24 24v88h8c13.3 0 24 10.7 24 24s-10.7 24-24 24H216c-13.3 0-24-10.7-24-24s10.7-24 24-24zm40-208a32 32 0 1 1 0 64 32 32 0 1 1 0-64z\"\/><\/svg><\/div><\/span><div class=\"stk-block-tab-labels__text has-text-align-left\"><span>Equipamiento<\/span><\/div><\/button><button class=\"stk-block-tabs__tab\" role=\"tab\"><span class=\"stk--svg-wrapper\"><div class=\"stk--inner-svg\"><svg style=\"height:0;width:0\"><defs><linearGradient id=\"linear-gradient-75e000b\" x1=\"0\" x2=\"100%\" y1=\"0\" y2=\"0\"><stop offset=\"0%\" style=\"stop-opacity:1;stop-color:var(--linear-gradient-75-e-000-b-color-1)\"><\/stop><stop offset=\"100%\" style=\"stop-opacity:1;stop-color:var(--linear-gradient-75-e-000-b-color-2)\"><\/stop><\/linearGradient><\/defs><\/svg><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 512 512\" aria-hidden=\"true\" width=\"32\" height=\"32\"><path d=\"M256 512A256 256 0 1 0 256 0a256 256 0 1 0 0 512zM216 336h24V272H216c-13.3 0-24-10.7-24-24s10.7-24 24-24h48c13.3 0 24 10.7 24 24v88h8c13.3 0 24 10.7 24 24s-10.7 24-24 24H216c-13.3 0-24-10.7-24-24s10.7-24 24-24zm40-208a32 32 0 1 1 0 64 32 32 0 1 1 0-64z\"\/><\/svg><\/div><\/span><div class=\"stk-block-tab-labels__text has-text-align-left\"><span>Servicios y Tarifas<\/span><\/div><\/button><button class=\"stk-block-tabs__tab\" role=\"tab\"><span class=\"stk--svg-wrapper\"><div class=\"stk--inner-svg\"><svg style=\"height:0;width:0\"><defs><linearGradient id=\"linear-gradient-75e000b\" x1=\"0\" x2=\"100%\" y1=\"0\" y2=\"0\"><stop offset=\"0%\" style=\"stop-opacity:1;stop-color:var(--linear-gradient-75-e-000-b-color-1)\"><\/stop><stop offset=\"100%\" style=\"stop-opacity:1;stop-color:var(--linear-gradient-75-e-000-b-color-2)\"><\/stop><\/linearGradient><\/defs><\/svg><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 512 512\" aria-hidden=\"true\" width=\"32\" height=\"32\"><path d=\"M256 512A256 256 0 1 0 256 0a256 256 0 1 0 0 512zM216 336h24V272H216c-13.3 0-24-10.7-24-24s10.7-24 24-24h48c13.3 0 24 10.7 24 24v88h8c13.3 0 24 10.7 24 24s-10.7 24-24 24H216c-13.3 0-24-10.7-24-24s10.7-24 24-24zm40-208a32 32 0 1 1 0 64 32 32 0 1 1 0-64z\"\/><\/svg><\/div><\/span><div class=\"stk-block-tab-labels__text has-text-align-left\"><span>Funcionamiento<\/span><\/div><\/button><\/div><\/div>\n\n\n\n<div class=\"wp-block-stackable-tab-content stk-block-tab-content stk-block stk-b4c5087\" data-block-id=\"b4c5087\"><div class=\"stk-row stk-inner-blocks stk-block-content stk-content-align stk-b4c5087-column\">\n<div class=\"wp-block-stackable-column stk-block-column stk-column stk-block stk-b8a50db\" data-v=\"4\" role=\"tabpanel\" data-block-id=\"b8a50db\"><div class=\"stk-column-wrapper stk-block-column__content stk-container stk-b8a50db-container stk--no-background stk--no-padding\"><div class=\"stk-block-content stk-inner-blocks stk-b8a50db-inner-blocks\">\n<p class=\"wp-block-paragraph\">La Microscop\u00eda Confocal supone un avance en el campo de la Microscop\u00eda \u00d3ptica de Fluorescencia y constituye una nueva y poderosa herramienta para examinar las estructuras celulares y sus funciones. El Microscopio Confocal permite el estudio de muestras con marcaje fluorescente, obteni\u00e9ndose secciones \u00f3pticas de las mismas mediante la excitaci\u00f3n de la muestra punto a punto por medio de un barrido l\u00e1ser.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">El microscopio Confocal permite que solo observemos el plano que est\u00e1 situado en el punto de foco, eliminando de forma \u00f3ptica a trav\u00e9s de un diafragma o \u201cpinhole\u201d, la luz proveniente de los planos que est\u00e1n fuera de foco.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">El equipo nos posibilita el estudio tridimensional de las muestras, incluyendo su interior, y en determinados materiales permite la obtenci\u00f3n de im\u00e1genes de la superficie de la muestra mediante reflexi\u00f3n<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Tambi\u00e9n se aplica para el estudio de muestras in vivo a lo largo de una secuencia temporal o para la colocalizaci\u00f3n de distintos marcadores en una regi\u00f3n concreta. El Microscopio est\u00e1 dotado con un sistema de detecci\u00f3n espectral por lo que permite obtener las curvas de autofluorescencia de diferentes muestras, los espectros de emisi\u00f3n de los fluor\u00f3foros si se desconocen y, eliminar o minimizar problemas de solapamiento de espectros. Tambi\u00e9n se pueden aplicar t\u00e9cnicas como FRET, FRAP, FLIP\u2026<br>Como resultado del desarrollo de la Microscop\u00eda Confocal, y de los m\u00e9todos digitales de an\u00e1lisis de im\u00e1genes, es posible actualmente abordar cuestiones relativas a las relaciones estructura-funci\u00f3n en los seres vivos, que anteriormente eran inabarcables.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Tambi\u00e9n se aplica para el estudio de muestras in vivo a lo largo de una secuencia temporal o para la colocalizaci\u00f3n de distintos marcadores en una regi\u00f3n concreta.<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"453\" height=\"187\" src=\"https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopia-1.jpg\" alt=\"\" class=\"wp-image-7101\" title=\"\" srcset=\"https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopia-1.jpg 453w, https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopia-1-300x124.jpg 300w, https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopia-1-18x7.jpg 18w\" sizes=\"auto, (max-width: 453px) 100vw, 453px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"315\" height=\"162\" src=\"https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopia-2.jpg\" alt=\"\" class=\"wp-image-7102\" title=\"\" srcset=\"https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopia-2.jpg 315w, https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopia-2-18x9.jpg 18w, https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopia-2-300x154.jpg 300w\" sizes=\"auto, (max-width: 315px) 100vw, 315px\" \/><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/investigacionpuertadehierro.com\/unidad-de-microscopia-confocal\/#tab-4af59ed0beac4eb4c8c\"><\/a><\/p>\n<\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-stackable-column stk-block-column stk-column stk-block stk-502b907\" data-v=\"4\" role=\"tabpanel\" data-block-id=\"502b907\"><div class=\"stk-column-wrapper stk-block-column__content stk-container stk-502b907-container stk--no-background stk--no-padding\"><div class=\"stk-block-content stk-inner-blocks stk-502b907-inner-blocks\">\n<p class=\"wp-block-paragraph\">La Unidad de Microscop\u00eda \u00f3ptica Avanzada y Confocal del IDIPHISA est\u00e1 equipada por:.<\/p>\n\n\n\n<div class=\"wp-block-stackable-columns alignwide stk-block-columns stk-block stk-2a72b54\" data-block-id=\"2a72b54\"><style>.stk-2a72b54 .stk--block-align-2a72b54{align-items:center !important;}<\/style><div class=\"stk-row stk-inner-blocks has-text-align-left stk--block-align-2a72b54 stk-block-content stk-content-align stk-2a72b54-column alignwide\">\n<div class=\"wp-block-stackable-column stk-block-column stk-column stk-block stk-d4d2385\" data-v=\"4\" data-block-id=\"d4d2385\"><div class=\"stk-column-wrapper stk-block-column__content stk-container stk-d4d2385-container stk--no-background stk--no-padding\"><div class=\"stk-block-content stk-inner-blocks stk-d4d2385-inner-blocks\">\n<p class=\"wp-block-paragraph\"><strong>Microscopio Confocal LSM90 con airysan 2,0 (m\u00f3dulo super resoluci\u00f3n)<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Con platina motorizada ,4 l\u00edneas de l\u00e1ser de estado s\u00f3lido, directamente conectadas a la unidad de escaneo para evitar alineamientos, con las potencias indicadas:<\/p>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<ul class=\"wp-block-list\">\n<li>L\u00e1ser 405 nm (15 mw)L\u00e1ser 488 nm (25 mw)<\/li>\n\n\n\n<li>L\u00e1ser 561 nm (25 mw)<\/li>\n\n\n\n<li>L\u00e1ser 640 nm (15 mw)<\/li>\n<\/ul>\n<\/div>\n\n\n\n<p class=\"wp-block-paragraph\">4 detectores :<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>2 detectores MA-PMT para fluorescencia con rango din\u00e1mico de 16 bit Airyscan<\/li>\n\n\n\n<li>2 de alta sensibilidad.<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">Aumento de resoluci\u00f3n y SNR 1 detector ESID para luz transmitida.<\/p>\n<\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-stackable-column stk-block-column stk-column stk-block stk-a8c6231\" data-v=\"4\" data-block-id=\"a8c6231\"><div class=\"stk-column-wrapper stk-block-column__content stk-container stk-a8c6231-container stk--no-background stk--no-padding\"><div class=\"stk-block-content stk-inner-blocks stk-a8c6231-inner-blocks\">\n<figure class=\"wp-block-image aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"920\" height=\"690\" src=\"https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopio-Confocal-LSM90.jpg\" alt=\"\" class=\"wp-image-7090\" srcset=\"https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopio-Confocal-LSM90.jpg 920w, https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopio-Confocal-LSM90-300x225.jpg 300w, https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopio-Confocal-LSM90-768x576.jpg 768w, https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopio-Confocal-LSM90-16x12.jpg 16w\" sizes=\"auto, (max-width: 920px) 100vw, 920px\" \/><\/figure>\n<\/div><\/div><\/div>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-stackable-columns alignwide stk-block-columns stk-block stk-ba0f7a0\" data-block-id=\"ba0f7a0\"><style>.stk-ba0f7a0 .stk--block-align-ba0f7a0{align-items:center !important;}<\/style><div class=\"stk-row stk-inner-blocks has-text-align-left stk--block-align-ba0f7a0 stk-block-content stk-content-align stk-ba0f7a0-column alignwide\">\n<div class=\"wp-block-stackable-column stk-block-column stk-column stk-block stk-b440e0a\" data-v=\"4\" data-block-id=\"b440e0a\"><div class=\"stk-column-wrapper stk-block-column__content stk-container stk-b440e0a-container stk--no-background stk--no-padding\"><div class=\"stk-block-content stk-inner-blocks stk-b440e0a-inner-blocks\">\n<p class=\"wp-block-paragraph\"><strong>Microscopio Confocal Leica SP5<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">con tres l\u00edneas l\u00e1ser, est\u00e1 optimizado para la adquisici\u00f3n de im\u00e1genes a partir de muestras, tanto fijadas como in vivo, que no requieran de unas condiciones ambientales espec\u00edficas. En este sistema contamos con 3 l\u00edneas l\u00e1ser de excitaci\u00f3n:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>L\u00e1ser de Arg\u00f3n, que excitar\u00e1 a las siguientes longitudes de onda; 458, 477, 488, 496 y 514 nm.<\/li>\n\n\n\n<li>L\u00e1ser de Helio\/Ne\u00f3n (He\/Ne), que excita a 543 nm.<\/li>\n\n\n\n<li>L\u00e1ser de Helio\/Ne\u00f3n (He\/Ne) que excita a 633 nm.<\/li>\n<\/ul>\n<\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-stackable-column stk-block-column stk-column stk-block stk-e645493\" data-v=\"4\" data-block-id=\"e645493\"><div class=\"stk-column-wrapper stk-block-column__content stk-container stk-e645493-container stk--no-background stk--no-padding\"><div class=\"stk-block-content stk-inner-blocks stk-e645493-inner-blocks\">\n<figure class=\"wp-block-image aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"848\" height=\"561\" src=\"https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopio-Confocal-Leica-SP5.jpg\" alt=\"\" class=\"wp-image-7091\" srcset=\"https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopio-Confocal-Leica-SP5.jpg 848w, https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopio-Confocal-Leica-SP5-300x198.jpg 300w, https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopio-Confocal-Leica-SP5-18x12.jpg 18w, https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopio-Confocal-Leica-SP5-768x508.jpg 768w\" sizes=\"auto, (max-width: 848px) 100vw, 848px\" \/><\/figure>\n<\/div><\/div><\/div>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-stackable-columns alignwide stk-block-columns stk-block stk-b231c25\" data-block-id=\"b231c25\"><style>.stk-b231c25 .stk--block-align-b231c25{align-items:center !important;}<\/style><div class=\"stk-row stk-inner-blocks has-text-align-left stk--block-align-b231c25 stk-block-content stk-content-align stk-b231c25-column alignwide\">\n<div class=\"wp-block-stackable-column stk-block-column stk-column stk-block stk-63c4819\" data-v=\"4\" data-block-id=\"63c4819\"><div class=\"stk-column-wrapper stk-block-column__content stk-container stk-63c4819-container stk--no-background stk--no-padding\"><div class=\"stk-block-content stk-inner-blocks stk-63c4819-inner-blocks\">\n<p class=\"wp-block-paragraph\"><strong>Microscopio Wide Field Thunder LEICA<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Microscopio Leica 5 LEDS<\/li>\n\n\n\n<li>Microscopio invertido con aumentos 5x, 20x, 40x, 63x con contraste fase y campo claro.<\/li>\n\n\n\n<li>Platina motorizada, y incubador CO2 , Control Temperaturac\u00e1mara sCMOS (Leica K5)<\/li>\n<\/ul>\n<\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-stackable-column stk-block-column stk-column stk-block stk-d850dbe\" data-v=\"4\" data-block-id=\"d850dbe\"><div class=\"stk-column-wrapper stk-block-column__content stk-container stk-d850dbe-container stk--no-background stk--no-padding\"><div class=\"stk-block-content stk-inner-blocks stk-d850dbe-inner-blocks\">\n<figure class=\"wp-block-image aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"515\" height=\"386\" src=\"https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopio-Wide-Field-Thunder-LEICA-1.jpg\" alt=\"\" class=\"wp-image-7092\" srcset=\"https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopio-Wide-Field-Thunder-LEICA-1.jpg 515w, https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopio-Wide-Field-Thunder-LEICA-1-300x225.jpg 300w, https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopio-Wide-Field-Thunder-LEICA-1-16x12.jpg 16w\" sizes=\"auto, (max-width: 515px) 100vw, 515px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"523\" height=\"392\" src=\"https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopio-Wide-Field-Thunder-LEICA-2.jpg\" alt=\"\" class=\"wp-image-7093\" srcset=\"https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopio-Wide-Field-Thunder-LEICA-2.jpg 523w, https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopio-Wide-Field-Thunder-LEICA-2-300x225.jpg 300w, https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/10\/Microscopio-Wide-Field-Thunder-LEICA-2-16x12.jpg 16w\" sizes=\"auto, (max-width: 523px) 100vw, 523px\" \/><\/figure>\n<\/div><\/div><\/div>\n<\/div><\/div>\n\n\n\n<p class=\"wp-block-paragraph\">La unidad tambi\u00e9n cuenta con:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>un&nbsp;<strong>citospin de Shandon<\/strong>&nbsp;para poder pasar las c\u00e9lulas crecidas en suspensi\u00f3n a portaobjetos y tambi\u00e9n se encuentran en la Unidad 2 microscopios de luz transmitida.<\/li>\n\n\n\n<li>un&nbsp;<strong>microscopio de luz transmitida Leica<\/strong>&nbsp;conectado a una c\u00e1mara Leica MC190HD y un ordenador que permite la adquisici\u00f3n de im\u00e1genes de campo claro como Hematoxilinas, y otras tinciones<\/li>\n<\/ul>\n<\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-stackable-column stk-block-column stk-column stk-block stk-559faaf\" data-v=\"4\" role=\"tabpanel\" data-block-id=\"559faaf\"><div class=\"stk-column-wrapper stk-block-column__content stk-container stk-559faaf-container stk--no-background stk--no-padding\"><div class=\"stk-block-content stk-inner-blocks stk-559faaf-inner-blocks\">\n<div id=\"funcionamiento\"><\/div><h5 id=\"gspb_heading-id-gsbp-c42f8ef\" class=\"gspb_heading gspb_heading-id-gsbp-c42f8ef \">CARTERA DE SERVICIOS<\/h5>\n\n\n\n<p class=\"wp-block-paragraph\">El servicio que ofrece la Unidad de Microscop\u00eda Confocal es el de asistir a los grupos de investigaci\u00f3n del centro o a investigadores visitantes, en el proceso de obtenci\u00f3n de im\u00e1genes utilizando t\u00e9cnicas de Microscop\u00eda Confocal, planteamiento del proyecto, preparaci\u00f3n de muestras, selecci\u00f3n de marcadores, procesamiento de im\u00e1genes, cuantificaci\u00f3n mediante programas inform\u00e1ticos espec\u00edficos, transferencia de datos, presentaci\u00f3n y almacenamiento de im\u00e1genes.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">El Servicio mantiene un stock bastante amplio de fluorocromos que adquiere sobre la base de las necesidades de los usuarios y facilita al\u00edcuotas a bajo costo.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>CARTERA DE SERVICIOS<\/strong><\/p>\n\n\n\n<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<ul class=\"wp-block-list\">\n<li>Preparaci\u00f3n de muestras.<\/li>\n\n\n\n<li>Tratamiento de la muestra para Microscop\u00eda de Fluorescencia<\/li>\n\n\n\n<li>Inmunofluorescencia.<\/li>\n\n\n\n<li>Visualizaci\u00f3n de muestras y captura de im\u00e1genes en Microscopio Confocal.<\/li>\n\n\n\n<li>An\u00e1lisis se datos, cuantificaci\u00f3n, procesamiento de im\u00e1genes, preparaci\u00f3n de planchas para publicaciones.<\/li>\n\n\n\n<li>Servicios de asesor\u00eda.<\/li>\n<\/ul>\n<\/div>\n\n\n\n<div id=\"funcionamiento\"><\/div><h5 id=\"gspb_heading-id-gsbp-dea2a1a\" class=\"gspb_heading gspb_heading-id-gsbp-dea2a1a \">TARIFAS<\/h5>\n\n\n\n<div class=\"wp-block-greenshift-blocks-iconlist gspb_iconsList gspb_iconsList-id-gsbp-8acfc32\" id=\"gspb_iconsList-id-gsbp-8acfc32\"><div class=\"gspb_iconsList__item\" data-id=\"0\"><a class=\"gspb_iconsList__link\" href=\"https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/08\/TARIFAS-UATIs-2026.pdf\" target=\"_blank\" rel=\"noopener\"><\/a><img decoding=\"async\" src=\"https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/03\/pdf.png\"\/><span class=\"gspb_iconsList__item__text\"><strong><em><a href=\"https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/08\/TARIFAS-UATIs-2026.pdf\" data-type=\"link\" data-id=\"https:\/\/investigacionpuertadehierro.com\/wp-content\/uploads\/2026\/08\/TARIFAS-UATIs-2026.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Tarifas vigentes<\/a><\/em><\/strong><\/span><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-stackable-column stk-block-column stk-column stk-block stk-6714c39\" data-v=\"4\" role=\"tabpanel\" data-block-id=\"6714c39\"><div class=\"stk-column-wrapper stk-block-column__content stk-container stk-6714c39-container stk--no-background stk--no-padding\"><div class=\"stk-block-content stk-inner-blocks stk-6714c39-inner-blocks\">\n<p class=\"wp-block-paragraph\">El responsable de la Unidad controlar\u00e1 y manejar\u00e1 el microscopio y el software. En ning\u00fan momento el usuario podr\u00e1 encender o manejar el Microscopio Confocal ya que dada su complejidad se requiere personal cualificado y formado espec\u00edficamente para su uso.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Las transferencias de im\u00e1genes se realizar\u00e1n mediante la grabaci\u00f3n de \u00e9stas en un soporte inform\u00e1tico del tipo de discos compactos.<\/p>\n\n\n\n<div id=\"funcionamiento\"><\/div><h5 id=\"gspb_heading-id-gsbp-47f00c3\" class=\"gspb_heading gspb_heading-id-gsbp-47f00c3 \">PERSONAL<\/h5>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Coordinadora Cient\u00edfica<\/strong>: Dra. Margarita S\u00e1nchez-Beato<br><\/li>\n\n\n\n<li><strong>Responsable Cient\u00edfica de la Unidad<\/strong>: Dra. Maria Jos\u00e9 Coronado<br>Tfno: 91 191 67 56<\/li>\n\n\n\n<li>E-mail: <a href=\"mailto:cultivoscelulares@idiphim.org\">confocal@idiphim.org<\/a><\/li>\n\n\n\n<li><strong>Ubicaci\u00f3n<\/strong>: 3\u00aa planta del \u00e1rea de Investigaci\u00f3n del Instituto de Investigaci\u00f3n del Hospital Puerta de Hierro Majadahonda<\/li>\n<\/ul>\n\n\n\n<div id=\"funcionamiento\"><\/div><h5 id=\"gspb_heading-id-gsbp-81c23ca\" class=\"gspb_heading gspb_heading-id-gsbp-81c23ca \">RESERVA DEL SERVICIO INVESTIGADORES PUERTA DE HIERRO:<\/h5>\n\n\n\n<p class=\"wp-block-paragraph\">Para consultar tarifas y utilizar el servicio debe hacer una reserva previa en la&nbsp;<a href=\"https:\/\/sites.google.com\/a\/idiphim.org\/confocal\/\">web de la Unidad&nbsp;<\/a>(requiere clave de usuario interno).<\/p>\n\n\n\n<div id=\"funcionamiento\"><\/div><h5 id=\"gspb_heading-id-gsbp-7248d66\" class=\"gspb_heading gspb_heading-id-gsbp-7248d66 \">RESERVA DEL SERVICIO USUARIOS EXTERNOS:<\/h5>\n\n\n\n<p class=\"wp-block-paragraph\">Puede solicitar el servicio contactando previamente por email con la responsable de la Unidad.<\/p>\n<\/div><\/div><\/div>\n<\/div><\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Descripci\u00f3n Equipamiento Servicios y Tarifas Funcionamiento La Microscop\u00eda Confocal supone un avance en el campo de la Microscop\u00eda \u00d3ptica de Fluorescencia y constituye una nueva y poderosa herramienta para examinar las estructuras celulares y sus funciones. El Microscopio Confocal permite el estudio de muestras con marcaje fluorescente, obteni\u00e9ndose secciones \u00f3pticas de las mismas mediante la [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","_gspb_post_css":"#gspb_row-id-gsbp-3c65ca7{justify-content:space-between;margin-top:0;margin-bottom:0;display:flex;flex-wrap:wrap}#gspb_row-id-gsbp-3c65ca7>.gspb_row__content{display:flex;justify-content:space-between;margin:0 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